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Turkish Journal of Biology

Abstract

Background/aim: Head and neck cancer (HNC) is one of the most prevalent causes of death worldwide, and the discovery of anticancer agents for its prevention is very important. Pterostilbene (PS) is a trans-stilbene reported to be beneficial in managing various cancers. The objective of the study was to evaluate the cytotoxic, anti-proliferative, pro-apoptotic and anti-migrative effect of PS on HEp-2, SCC-90, SCC-9, FaDu and Detroit-551 cell lines. Materials and Methods: MTT and live/dead assays were employed to assess cell-viability, while cell migration test was performed to evaluate wound healing capacity. The mRNA, protein and intracellular expression level of CASP-3, BAX and BCL-2 genes were evaluated by real-time PCR, western-blottig and immunofluorescence staining. Annexin V-PI staining was conducted to assess the amount of viable, apoptotic and necrotic cells.Results: The results revealed that PS displayed cytotoxic, antiproliferative activity in a dose dependent manner in HNC cells by up-regulation of CASP-3, BCL-2 but down-regulation of BCL-2 in the apoptotic pathway. The pro-apoptotic properties were confirmed by Annexin-V-IP results. Moreover, PS displayed a significant important suppressive efficacy on the migration capacity of HNC cells.Conclusion: The present study gives proof that PS has the prospective to be improved as an attractive anticancer agent aganist HNC following advanced studies.

Author ORCID Identifier

SİBEL ÖZDAŞ: 0000-0003-4610-2785

TALİH ÖZDAŞ: 0000-0003-3651-1892

İPEK CANATAR: 0000-0001-9448-8112

ERDEM KAYPAK: 0000-0002-8444-6530

DOI

10.55730/1300-0152.2708

Keywords

anti-cancer, apoptosis, cytotoxicity, Head and neck cancer, pterostilbene

First Page

319

Last Page

337

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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