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Turkish Journal of Biology

Abstract

Background/aim: Aging, a multifaceted biological process, leading to diminished physical performance, especially in older adults with diabetes, where a mismatch between biological and chronological age is noticeable. Numerous studies have demonstrated that diabetes accelerates aging at both cellular and organ levels. Notable aging markers are TERT, related to telomere length, and COL1A1, a key component of skin collagen. Additionally, age-related methylation increases, as revealed through methylation analysis, illuminate aspects of aging. However, the detailed interplay between aging and diabetes, particularly regarding methylation, remains underexplored and warrants further study to elucidate their biological links.Materials and methods: In this research, we elucidate the modulatory influence of diabetes on the aging process, focusing specifically on the modifications in the TERT within the kidney and the COL1A1 within the skin by employing mice with Swiss Webster strain as diabetes model. Specimens were categorized into three distinct chronological cohorts: chronologically young (16 weeks) (N=5), chronologically old (40 weeks) (N=5), and a periodically assessed group (16 weeks) (N=30), within which five mice were systematically sacrificed on a weekly basis.Results: Our findings reveal a marked impact of diabetes on the methylation statuses of TERT and COL1A1, characterized by an elevation in methylation levels within the periodic group (1st-6th weeks) and a concomitant, progressive attenuation in the expression of TERT and COL1A1 genes.Conclusion: The observed alterations in the methylation levels of TERT and COL1A1 propound the hypothesis that diabetes potentially expedites the aging process, concomitantly impinging on the production of TERT and COL1A, ostensibly through the mechanism of promoter gene hypermethylation.

Author ORCID Identifier

JESSICA LIAMRY: 0009-0005-1490-6561

FARIZKY MARTRIANO HUMARDANI: 0000-0003-0487-6926

GIOVANI CHANDRA: 0009-0007-3092-6380

LISA THALIA MULYANATA: 0009-0009-0700-3101

TJIE KOK: 0000-0003-3819-9599

FENNY IRAWATI: 0000-0002-8364-4885

HIKMAWAN SULISTOMO: 0000-0002-5343-6428

CHRISTOPH REICHETZEDER: 0000-0002-2219-5529

SULISTYO EMANTOKO DWI PUTRA: 0000-0001-8469-9688

DOI

10.55730/1300-0152.2701

Keywords

AgingCOL1A1diabetesmethylationTERT

First Page

257

Last Page

266

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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