Astragaloside IV improves renal function and alleviates renal damage and inflammation in rats with chronic glomerulonephritis

Authors

DONG ZHANG
ZONGYING LI
YUAN GAO
HAILING SUN

DOI

10.55730/1300-0152.2641

Abstract

From Astragalus membranaceus (Fisch.) Bge.var. mongholicus (Bge.) Hsiao, astragaloside IV (AS-IV), a saponin can be purified and is considered traditional Chinese medicine. The purpose of this study was to evaluate the AS-IV-mediated mechanism on chronic glomerulonephritis (CGN). A cationic bovine serum albumin-induced CGN rat model was established and 10, 15, or 20 mg/kg of AS-IV was administered to measure renal function and inflammatory infiltration. Influences of AS-IV on proliferation, cell cycle, and inflammation of LPS-induced rat mesangial cells (RMCs) were determined. The results demonstrated that AS-IV alleviated renal dysfunction, renal lesions, and inflammation in CGN rats. AS-IV prolonged the G0-G1 phase, shortened the S phase, and inhibited cell proliferation and inflammation in RMCs. AS-IV can promote miR-181d-5p expression to inhibit CSF1. miR-181d-5p promotion or CSF1 suppression could further enhance the therapeutic role of AS-IV in CGN rats, while miR-181d-5p silencing or CSF1 overexpression abolished the effect of AS-IV. In conclusion, AS-IV by mediating the miR-181d-5p/CSF1 axis protects against CGN.

Keywords

Astragaloside IV, miR-181d-5p, CSF1, chronic glomerulonephritis, kidney function, inflammation

First Page

61

Last Page

73

Recommended Citation

ZHANG, DONG; LI, ZONGYING; GAO, YUAN; and SUN, HAILING (2023) "Astragaloside IV improves renal function and alleviates renal damage and inflammation in rats with chronic glomerulonephritis," Turkish Journal of Biology: Vol. 47: No. 1, Article 6. https://doi.org/10.55730/1300-0152.2641
Available at: https://journals.tubitak.gov.tr/biology/vol47/iss1/6