Astragaloside IV improves renal function and alleviates renal damage and inflammation in rats with chronic glomerulonephritis

Authors

DONG ZHANG
ZONGYING LI
YUAN GAO
HAILING SUN

Abstract

From Astragalus membranaceus (Fisch.) Bge.var. mongholicus (Bge.) Hsiao, astragaloside IV (AS-IV), a saponin can be purified and is considered traditional Chinese medicine. The purpose of this study was to evaluate the AS-IV-mediated mechanism on chronic glomerulonephritis (CGN). A cationic bovine serum albumin-induced CGN rat model was established and 10, 15, or 20 mg/kg of AS-IV was administered to measure renal function and inflammatory infiltration. Influences of AS-IV on proliferation, cell cycle, and inflammation of LPS-induced rat mesangial cells (RMCs) were determined. The results demonstrated that AS-IV alleviated renal dysfunction, renal lesions, and inflammation in CGN rats. AS-IV prolonged the G0-G1 phase, shortened the S phase, and inhibited cell proliferation and inflammation in RMCs. AS-IV can promote miR-181d-5p expression to inhibit CSF1. miR-181d-5p promotion or CSF1 suppression could further enhance the therapeutic role of AS-IV in CGN rats, while miR-181d-5p silencing or CSF1 overexpression abolished the effect of AS-IV. In conclusion, AS-IV by mediating the miR-181d-5p/CSF1 axis protects against CGN.

DOI

10.55730/1300-0152.2641

Keywords

Astragaloside IV, miR-181d-5p, CSF1, chronic glomerulonephritis, kidney function, inflammation

First Page

61

Last Page

73

Recommended Citation

ZHANG, DONG; LI, ZONGYING; GAO, YUAN; and SUN, HAILING (2023) "Astragaloside IV improves renal function and alleviates renal damage and inflammation in rats with chronic glomerulonephritis," Turkish Journal of Biology: Vol. 47: No. 1, Article 6. https://doi.org/10.55730/1300-0152.2641
Available at: https://journals.tubitak.gov.tr/biology/vol47/iss1/6