Turkish Journal of Biology
VARS1 Mutations Associated with Neurodevelopmental Disorder Are Located on A Short Amino Acid Stretch of The Anticodon-Binding Domain
Majority of 37 human aminoacyl tRNA synthetases have been incriminated in diverse, mostly recessive, genetic diseases. In accordance with this, we uncovered a novel homozygous valyl-tRNA synthetase 1 (VARS1) gene variant, leading to p.T1068M mutation. As in the previously reported VARS1 mutations, the affected individual harboring p.T1068M was experiencing a neurodevelopmental disorder with intractable seizures, psychomotor retardation, and microcephaly. To link this phenotypic outcome with the observed genotype, we structurally modeled human VARS1 and interpreted p.T1068M within the spatial distribution of previously reported VARS1 variants. As a result, we uncovered that p.T1068M is clustered with three other pathogenic mutations in a 15 amino acid long stretch of the VARS1 anticodon-binding domain. While forming a helix-turn-helix motif within the anticodon-binding domain, this stretch harbors one-fourth of the reported VARS1 mutations. Here, we propose that these clustered mutations can destabilize the interactions between the anticodon-binding and the tRNA synthetase domains and thus hindering the optimal enzymatic activity of VARS1. We expect that the depiction of this mutation cluster will pave the way for the development of drugs, capable of alleviating the functional impact of these mutations.
VARS1, whole exome sequencing, protein stability, structural modeling, mutation modeling, psychomotor retardation
HIZ, AYŞE SEMRA; KILIÇ, SEVAL; BADEMCİ, GÜNEY; KARAKULAK, TÜLAY; ERDOĞAN, AYBİKE; YÜCEL, BURCU ÖZDEN; YAZICIOĞLU, ÇİĞDEM ERESEN; BAĞRIYANIK, ŞERİFE ESRA ERDAL; YİŞ, ULUÇ; TEKİN, MUSTAFA; KARAKÜLAH, GÖKHAN; EREK, EZGİ KARACA; and ÖZTÜRK, MEHMET
"VARS1 Mutations Associated with Neurodevelopmental Disorder Are Located on A Short Amino Acid Stretch of The Anticodon-Binding Domain,"
Turkish Journal of Biology: Vol. 46:
6, Article 3.
Available at: https://journals.tubitak.gov.tr/biology/vol46/iss6/3