SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients

Authors

CİHAN TAŞTAN
BULUT YURTSEVER
GÖZDE KARAKUŞ
DERYA DİLEK KANÇAĞI
SEVDA DEMİR
SELEN ABANUZ
UTKU SEYİS
MÜLAZİM YILDIRIM
RECAİ KUZAY
ÖMER ELİBOL
SERAP ARBAK
MERVE AÇIKEL ELMAS
SELÇUK BİRDOĞAN
OSMAN UĞUR SEZERMAN
AYŞE SESİN KOCAGÖZ

Abstract

The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.

DOI

10.3906/biy-2004-113

Keywords

COVID-19, SARS-CoV-2, coronavirus

First Page

192

Last Page

202

Recommended Citation

TAŞTAN, CİHAN; YURTSEVER, BULUT; KARAKUŞ, GÖZDE; KANÇAĞI, DERYA DİLEK; DEMİR, SEVDA; ABANUZ, SELEN; SEYİS, UTKU; YILDIRIM, MÜLAZİM; KUZAY, RECAİ; ELİBOL, ÖMER; ARBAK, SERAP; ELMAS, MERVE AÇIKEL; BİRDOĞAN, SELÇUK; SEZERMAN, OSMAN UĞUR; and KOCAGÖZ, AYŞE SESİN (2020) "SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients," Turkish Journal of Biology: Vol. 44: No. 7, Article 8. https://doi.org/10.3906/biy-2004-113
Available at: https://journals.tubitak.gov.tr/biology/vol44/iss7/8