A magnetic affinity approach to identify plant GABA-binding proteins

Authors

JIE ZOU
JINGZHE GUO
SHISHENG LI

Abstract

In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.

DOI

10.3906/biy-1901-79

Keywords

GABA, receptor, magnetic bead, signaling, pollen

First Page

246

Last Page

255

Recommended Citation

ZOU, JIE; GUO, JINGZHE; and LI, SHISHENG (2019) "A magnetic affinity approach to identify plant GABA-binding proteins," Turkish Journal of Biology: Vol. 43: No. 4, Article 3. https://doi.org/10.3906/biy-1901-79
Available at: https://journals.tubitak.gov.tr/biology/vol43/iss4/3