Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-Lhistidine/alginate microbeads by platelet-rich plasma

Authors

GÖKHAN DURUKSU
SELEN POLAT
LEYLA KAYİŞ
NUR EKİMCİ GÜRCAN
GÜLÇİN GACAR
YUSUFHAN YAZIR

DOI

10.3906/biy-1802-13

Abstract

Type 1 diabetes is clinically characterized as the loss of control of glucose homeostasis due to the reduced number of insulinproducing cells. Long-term glycemic control after implantation could be maintained by preserving the cell viability and tissue-specific functions during the process of microencapsulation. In this study, alginate solution was supplemented with platelet-rich plasma (PRP) to improve the viability and preserve the cell functions during the encapsulation of a beta cell line (BRIN-BD11). Cell viability was assessed and insulin secretion and insulin stimulation index were evaluated. The polymerization of alginate with PRP enhanced the viability up to 61% in the alginate microbeads. PRP supplementation to the alginate composition not only increased the number of viable cells by 1.95-fold, but the insulin secretion also improved by about 66%. The stimulation index, however, was not affected by the PRP supplementation.

Keywords

Calcium alginate microbeads, diabetes, droplet method, pancreatic beta cells, insulin

First Page

297

Last Page

306

Recommended Citation

DURUKSU, GÖKHAN; POLAT, SELEN; KAYİŞ, LEYLA; GÜRCAN, NUR EKİMCİ; GACAR, GÜLÇİN; and YAZIR, YUSUFHAN (2018) "Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-Lhistidine/alginate microbeads by platelet-rich plasma," Turkish Journal of Biology: Vol. 42: No. 4, Article 5. https://doi.org/10.3906/biy-1802-13
Available at: https://journals.tubitak.gov.tr/biology/vol42/iss4/5