Turkish Journal of Biology
DOI
10.3906/biy-1802-13
Abstract
Type 1 diabetes is clinically characterized as the loss of control of glucose homeostasis due to the reduced number of insulinproducing cells. Long-term glycemic control after implantation could be maintained by preserving the cell viability and tissue-specific functions during the process of microencapsulation. In this study, alginate solution was supplemented with platelet-rich plasma (PRP) to improve the viability and preserve the cell functions during the encapsulation of a beta cell line (BRIN-BD11). Cell viability was assessed and insulin secretion and insulin stimulation index were evaluated. The polymerization of alginate with PRP enhanced the viability up to 61% in the alginate microbeads. PRP supplementation to the alginate composition not only increased the number of viable cells by 1.95-fold, but the insulin secretion also improved by about 66%. The stimulation index, however, was not affected by the PRP supplementation.
Keywords
Calcium alginate microbeads, diabetes, droplet method, pancreatic beta cells, insulin
First Page
297
Last Page
306
Recommended Citation
DURUKSU, GÖKHAN; POLAT, SELEN; KAYİŞ, LEYLA; GÜRCAN, NUR EKİMCİ; GACAR, GÜLÇİN; and YAZIR, YUSUFHAN
(2018)
"Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-Lhistidine/alginate microbeads by platelet-rich plasma,"
Turkish Journal of Biology: Vol. 42:
No.
4, Article 5.
https://doi.org/10.3906/biy-1802-13
Available at:
https://journals.tubitak.gov.tr/biology/vol42/iss4/5