Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-Lhistidine/alginate microbeads by platelet-rich plasma

Authors

GÖKHAN DURUKSU
SELEN POLAT
LEYLA KAYİŞ
NUR EKİMCİ GÜRCAN
GÜLÇİN GACAR
YUSUFHAN YAZIR

Abstract

Type 1 diabetes is clinically characterized as the loss of control of glucose homeostasis due to the reduced number of insulinproducing cells. Long-term glycemic control after implantation could be maintained by preserving the cell viability and tissue-specific functions during the process of microencapsulation. In this study, alginate solution was supplemented with platelet-rich plasma (PRP) to improve the viability and preserve the cell functions during the encapsulation of a beta cell line (BRIN-BD11). Cell viability was assessed and insulin secretion and insulin stimulation index were evaluated. The polymerization of alginate with PRP enhanced the viability up to 61% in the alginate microbeads. PRP supplementation to the alginate composition not only increased the number of viable cells by 1.95-fold, but the insulin secretion also improved by about 66%. The stimulation index, however, was not affected by the PRP supplementation.

DOI

10.3906/biy-1802-13

Keywords

Calcium alginate microbeads, diabetes, droplet method, pancreatic beta cells, insulin

First Page

297

Last Page

306

Recommended Citation

DURUKSU, GÖKHAN; POLAT, SELEN; KAYİŞ, LEYLA; GÜRCAN, NUR EKİMCİ; GACAR, GÜLÇİN; and YAZIR, YUSUFHAN (2018) "Improvement of the insulin secretion from beta cells encapsulated in alginate/poly-Lhistidine/alginate microbeads by platelet-rich plasma," Turkish Journal of Biology: Vol. 42: No. 4, Article 5. https://doi.org/10.3906/biy-1802-13
Available at: https://journals.tubitak.gov.tr/biology/vol42/iss4/5