Effects of various preculture, pretreatment, and recovery conditions on the morphogenetic response of cryopreserved Lady Orange chrysanthemum shoot tips

Authors

DARIUSZ KULUS

Abstract

The aim of this research was to optimize the encapsulation-dehydration cryopreservation protocol of Lady Orange chrysanthemum explants on the preculture, pretreatment, and post-rewarming recovery steps. Shoot tips were precultured on MS medium with various abscisic acid concentrations (0-30 µM). Next, the encapsulated explants were osmotically dehydrated for 3 or 5 days and desiccated for 3-4 h and, after rewarming, they were subcultured on recovery media of various compositions (control and cytokinin/auxin-supplemented). A high explant survival rate, even up to 100%, was observed. The value of this parameter, however, changed depending on the post-rewarming culture duration. Moreover, not all the viable explants were capable of forming shoots. A lower ABA concentration (15 µM) during preculture and the presence of 4.65 µM kinetin in the post-rewarming recovery medium enhanced cryopreservation efficiency with a high survival rate and typical microshoot formation. A higher ABA concentration and the presence of 6-benzylaminopurine in the recovery medium resulted in shoot multiplication, abundant callus formation, and root formation inhibition.

DOI

10.3906/biy-1711-47

Keywords

Abscisic acid, dehydration, desiccation, encapsulation, liquid nitrogen, plant growth regulators, regeneration

First Page

76

Last Page

86

Recommended Citation

KULUS, DARIUSZ (2018) "Effects of various preculture, pretreatment, and recovery conditions on the morphogenetic response of cryopreserved Lady Orange chrysanthemum shoot tips," Turkish Journal of Biology: Vol. 42: No. 1, Article 8. https://doi.org/10.3906/biy-1711-47
Available at: https://journals.tubitak.gov.tr/biology/vol42/iss1/8