Turkish Journal of Biology
Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate
Abstract
The present study focused on the screening and optimization of biopolymer polyhydroxybutyrate (PHB) production by Bacillus spp. using cost-effective substrates. Among 602 local Bacillus isolates, Bacillus thuringiensis B417-5 produced the highest amount of PHB (2.278 g/L, 60.07% of dry cell weight, DCW). 1H NMR and FTIR analyses of the extracted polymer revealed the characteristic peaks of PHB. The optimization results showed that the highest PHB accumulation (2.768 g/L, 72.08% of DCW) was achieved when culturing B. thuringiensis B417-5 in a nitrogen-deficient medium containing 1% total sugar from sugarcane juice and 0.5% yeast extract, with a pH of 7.0 and an incubation temperature of 37 °C for 48 h. B. thuringiensis B417-5 can thus be considered a good candidate for large-scale production of PHB. We are reporting for the first time that sugarcane juice is a promising carbon source for economical PHB production by B. thuringiensis.
DOI
10.3906/biy-1704-13
Keywords
Bacillus thuringiensis, bioplastic, polyhydroxyalkanoate, polyhydroxybutyrate, sugarcane juice
First Page
992
Last Page
1002
Recommended Citation
THAMMASITTIRONG, ANON; SAECHOW, SUDARAT; and THAMMASITTIRONG, SUTTICHA NA-RANONG
(2017)
"Efficient polyhydroxybutyrate production from Bacillus thuringiensis using sugarcane juice substrate,"
Turkish Journal of Biology: Vol. 41:
No.
6, Article 14.
https://doi.org/10.3906/biy-1704-13
Available at:
https://journals.tubitak.gov.tr/biology/vol41/iss6/14
