N-Acetylglucoseamine modified alginate sponges as scaffolds for skin tissue engineering

Authors

MURAT DEMİRBİLEK
NELİSA TÜRKOĞLU
SELÇUK AKTÜRK

DOI

10.3906/biy-1704-31

Abstract

In the treatment of dermal wounds, wound-dressing materials prepared from natural mucopolysaccharides are widely used because of their advantages such as nonirritation, nontoxicity, and ease in topical application. In the present study, alginate hydrogels modified with N-acetyl glucose amine (NAG) were prepared as wound-dressing material. Physical, chemical, thermal, and mechanical properties of the hydrogels were studied. Cytotoxicity of the hydrogels on endothelial (HUVEC) and keratinocyte (HaCaT) cells were examined. Anti- and proinflammatory cytokine levels of human monocyte-macrophage cells (THP-1) stimulated with hydrogels were determined. According to the results, increasing the NAG concentration led to an increase in the swelling and nitrogen ratios in the hydrogels. Additionally, increasing the NAG concentration decreased elastic modulus and degradation time. Hydrogels were not cytotoxic on HaCaT and HUVEC cells. It stimulated IL-10 and TNF-alpha levels at a small rate.

Keywords

Biomaterials, alginate hydrogels, hyaluronic acid monomers, cytokines

First Page

796

Last Page

807

Recommended Citation

DEMİRBİLEK, MURAT; TÜRKOĞLU, NELİSA; and AKTÜRK, SELÇUK (2017) "N-Acetylglucoseamine modified alginate sponges as scaffolds for skin tissue engineering," Turkish Journal of Biology: Vol. 41: No. 5, Article 10. https://doi.org/10.3906/biy-1704-31
Available at: https://journals.tubitak.gov.tr/biology/vol41/iss5/10