Optimization, purification, and characterization of an extracellular antifungal protein from Serratia marcescens DT3 isolated from soil in Vietnam

Authors

TUYEN THI DO
THAO THI NGUYEN
THANH SY LE NGUYEN
HOANG THANH LE

DOI

10.3906/biy-1607-22

Abstract

Gram-negative Serratia marcescens is a potential biological control agent of fungal plant diseases in many tropical regions. Its antifungal activity is due to the production of prodigiosin, protease, chitinase, and other substances that act to weaken the fungal cell wall and cause lysis. In the following investigation, a novel extracellular protein with antifungal activity against Rhizoctonia solani and Fusarium oxysporum was optimally produced, purified, and characterized from a native Serratia marcescens DT3. Under optimal conditions (medium containing 1.25% peptone, 0.5% yeast extract; temperature of 28 °C; initial pH of 7; culture time of 28 h), the antifungal activity of the extracellular extract was increased by a factor of 1.6-2.9 in comparison with the initial condition. The purified protein had an apparent molecular mass of 55 kDa. Its antifungal activity was retained at 80 °C for 30 min and after having been treated with proteinase K (0.5-4 μg/mL). The results of protein identification using a MALDI-TOF/TOF mass spectrometer suggested that the purified protein is indeed a serine 3-dehydrogenase.

Keywords

Antifungal protein, Serratia marcescens DT3, serine 3-hydroxylase

First Page

448

Last Page

457

Recommended Citation

DO, TUYEN THI; NGUYEN, THAO THI; NGUYEN, THANH SY LE; and LE, HOANG THANH (2017) "Optimization, purification, and characterization of an extracellular antifungal protein from Serratia marcescens DT3 isolated from soil in Vietnam," Turkish Journal of Biology: Vol. 41: No. 3, Article 5. https://doi.org/10.3906/biy-1607-22
Available at: https://journals.tubitak.gov.tr/biology/vol41/iss3/5