The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche

Authors

NA LI
DANDAN YIN
HUIJIE ZHANG
JINMEI XU
FAN WEN
ZHENG LIU
YAOZHEN CHEN
NING AN
JIAJIA XIN
YAZHOU WANG
WEN YIN
XINGBIN HU

Abstract

The hematopoietic microenvironment regulates self-renewal and differentiation of hematopoietic stem cells. Mesenchymal stromal cells (MSCs) contribute to the niche and participate in supporting dendritic cell (DC) commitment in vitro and in vivo. However, due to MSCs being heterogenic, it is necessary to understand the function of the MSC subpopulation in modulating the DC commitment. The current study showed that one of the bone-related Sca1+ MSCs enhanced bone marrow cell differentiation into DCs by direct cell-to-cell contact. Furthermore, the expression of STAT3 and STAT5 genes decreased in supernatant cells after Sca1+ MSCs were cocultured with bone marrow cells. In contrast, the expression level of caspase-3 in cells increased. At the same time, the presentation of costimulatory molecules CD80/CD86 and the migration of activated DCs towards lymph nodes were augmented by Sca1+ MSCs. The results demonstrated that the Sca1+ MSC subpopulation promotes the differentiation of bone marrow cells into DCs by direct cell-to-cell contact via STAT3/STAT5 and caspase-3 signaling. Meanwhile, Sca1+ MSCs also mediate the activation and migration capabilities of DCs.

DOI

10.3906/biy-1510-81

Keywords

Caspase-3, development, dendritic cells, migration capability, Sca1+ mesenchymal stromal cells

First Page

58

Last Page

65

Recommended Citation

LI, NA; YIN, DANDAN; ZHANG, HUIJIE; XU, JINMEI; WEN, FAN; LIU, ZHENG; CHEN, YAOZHEN; AN, NING; XIN, JIAJIA; WANG, YAZHOU; YIN, WEN; and HU, XINGBIN (2017) "The Sca1+ mesenchymal stromal subpopulation promotesdendritic cell commitment in the niche," Turkish Journal of Biology: Vol. 41: No. 1, Article 7. https://doi.org/10.3906/biy-1510-81
Available at: https://journals.tubitak.gov.tr/biology/vol41/iss1/7