Cloning of two basic helix-loop-helix transcription factor genes from Tartary buckwheat (Fagopyrum tataricum) and their expression under abiotic stress

Authors

XIAO-PENG LUO
HAI-XIA ZHAO
JUN XUE
CHENG-LEI LI
HUI CHEN
SANG-UN PARK
QI WU

Abstract

Basic helix-loop-helix (bHLH) transcription factors are one of the most important transcription factors involved in abiotic stress tolerance in Tartary buckwheat. In this study, we successfully isolated two bHLH transcription factor genes, designated as FtbHLH1 and FtbHLH2, from Tartary buckwheat. Their encoding proteins were found to have a motif to interact with R2R3-MYB at the N-terminus and a typical bHLH motif at the C-terminus. Yeast one-hybrid assay proved that the two bHLH proteins exhibited obvious transcriptional activation activities. The abiotic stresses indicated that FtbHLH1 expression would be mainly affected by drought and UV-B, whereas FtbHLH2 expression would present a sensitive response to cold and UV-B. Additionally, FtbHLH2 showed a more intense response to jasmonic acid treatment than FtbHLH1.

DOI

10.3906/biy-1511-36

Keywords

Tartary buckwheat, RACE, bHLH genes, transcription activation, semiquantitative RT-PCR

First Page

1192

Last Page

1201

Recommended Citation

LUO, XIAO-PENG; ZHAO, HAI-XIA; XUE, JUN; LI, CHENG-LEI; CHEN, HUI; PARK, SANG-UN; and WU, QI (2016) "Cloning of two basic helix-loop-helix transcription factor genes from Tartary buckwheat (Fagopyrum tataricum) and their expression under abiotic stress," Turkish Journal of Biology: Vol. 40: No. 6, Article 8. https://doi.org/10.3906/biy-1511-36
Available at: https://journals.tubitak.gov.tr/biology/vol40/iss6/8