Cloning and abiotic stress resistance analyses of a new proline-glycine-alaninehistidine-rich protein gene from Ipomoea batatas (L.) Lam.

Authors

XIANG TAO
BIN YONG
HUAN-HUAN SHAO
XIN-RONG MA

Abstract

Sweet potato [Ipomoea batatas (L.) Lam.] is one of the top six most important crops in the world in terms of production. It has the capacity to tolerate marginal growing conditions better than most other crops. In this study, a proline- and glycine-rich protein encoding gene, IbPGAHRP1, was isolated and characterized from the transcriptome of sweet potato cultivar Xushu 18. Sequence analyses showed that proline, glycine, alanine, and histidine account for 62.6% of the total amino acid residues. Two proline-rich tandem repeats, an alanine-rich repeat, a histidine-rich tandem repeat, and five XYPP motifs were present in IbPGAHRP1. Sequence similarity analysis showed that IbPGAHRP1 shared 41% similarity with GmGPRP4, indicating that IbPGAHRP1 should be a GPRP-like protein and play an important role in stress resistance. RT-PCR results showed that IbPGAHRP1 was specifically expressed in the sweet potato flower. In vivo expression of IbPGAHRP1 demonstrated that it enhanced the cold and drought resistance in the yeast recombinant, but could not trigger the same changes in Escherichia coli. This study revealed that IbPGAHRP1 is a flower-specific gene and should play an important role in response to abiotic stresses.

DOI

10.3906/biy-1509-76

Keywords

Flower-specific, IbPGAHRP1, sweet potato, abiotic stress

First Page

1148

Last Page

1157

Recommended Citation

TAO, XIANG; YONG, BIN; SHAO, HUAN-HUAN; and MA, XIN-RONG (2016) "Cloning and abiotic stress resistance analyses of a new proline-glycine-alaninehistidine-rich protein gene from Ipomoea batatas (L.) Lam.," Turkish Journal of Biology: Vol. 40: No. 6, Article 3. https://doi.org/10.3906/biy-1509-76
Available at: https://journals.tubitak.gov.tr/biology/vol40/iss6/3