Preparation and characterization of acellular porcinepericardium for cardiovascular surgery

Authors

HA LE BAO TRAN
TRANG THI HUYEN DINH
MY THI NGOC NGUYEN
QUAN MINH TO
ANH THO TUAN PHAM

DOI

10.3906/biy-1510-44

Abstract

The aim of this study was to fabricate and characterize acellular porcine pericardium that can be used to patch cardiovascular repair. Porcine pericardial tissues were treated with 10 mM Tris-HCl and sodium dodecyl sulfate (SDS) at different concentrations and time intervals. The optimal decellularization protocol was determined according to elimination of cellular components and DNA via histology and DNA quantification, respectively. There were no significant changes in stress and fracture strain after decellularization. Liquid extracts from the decellularized pericardium caused no cytotoxicity towards human fibroblasts, were capable of supporting an appropriate attachment of human endothelial progenitor cells, and did not cause a local inflammatory effect after 30 days of transplantation in mice.

Keywords

Pericardium, decellularization, patch, cardiovascular, biocompatibility

First Page

1243

Last Page

1250

Recommended Citation

TRAN, HA LE BAO; DINH, TRANG THI HUYEN; NGUYEN, MY THI NGOC; TO, QUAN MINH; and PHAM, ANH THO TUAN (2016) "Preparation and characterization of acellular porcinepericardium for cardiovascular surgery," Turkish Journal of Biology: Vol. 40: No. 6, Article 13. https://doi.org/10.3906/biy-1510-44
Available at: https://journals.tubitak.gov.tr/biology/vol40/iss6/13