Insufficient targeting of the therapeutic genes to tumor cells is one of the major reasons for failure in cancer gene therapy. Mesenchymal stem cells (MSCs) seem to be a good candidate as a carrier for gene therapy because of its selective tumor tissue-homing properties. In the current study, we constructed baculoviral vectors (BVs) carrying cytosine deaminase (CD) (BV-CD) or green fluorescence protein (GFP) genes (BV-GFP) and tested the transduction efficiency of the vectors in tumor and mesenchymal stem cells. We also tested the in vivo efficacy of the BV-CD vector in a colon cancer model. Our results showed that the recombinant baculoviral vectors can efficiently transduce mammalian cells and express genes of interest. The BV-CD vector treatment caused significant in vitro cytotoxicity when used with 5-fluorocytosine. MSCs loaded with the BV-CD vector caused a significant delay in tumor growth and increased survival when compared to control and MSC alone treated groups bearing colon cancer. Our results show that the recombinant BV-CD vector could be used either alone or loaded into MSCs in the treatment of established tumors.
Gene therapy, mesenchymal stem cells, baculoviral vector, cancer
AKTAŞ, SEDEF HANDE; AKBULUT, HAKAN; ELÇİN, AYŞE ESER; PARMAKSIZ, MAHMUT; AKTAN, ARZU KESKİN; CİHAN, ARZU ÇÖLERİ; ELÇİN, YAŞAR MURAT; and İÇLİ, FİKRİ
"Baculoviral vector loaded mesenchymal stem cells as efficient gene therapy tools for cancer treatment,"
Turkish Journal of Biology: Vol. 40:
5, Article 17.
Available at: https://journals.tubitak.gov.tr/biology/vol40/iss5/17