Turkish Journal of Biology
Abstract
Salinity is among the significant abiotic stresses adversely affecting plant development and yield for a wide range of crops. For quantitative and qualitative expression studies of proton gradient regulator 5 (PGR5) under salt stress, differential display RT-PCR and rapid amplification of cDNA ends (RACE) were performed on salt-stress responsive Gossypium arboreum. Alignment of genomic and cDNA sequences revealed that the GPGR5 gene comprises a single open reading frame of 96 amino acids and contains no introns. Alignment of cotton GPGR5 complete amino acid sequence with PGR5 from other plants revealed the following identities: Gossypium raimondii (97%), Amaranthus hybridus (72%), Vitis vinifera (69%), Medicago truncatula (68%), Cucumis melo (62%), Arabidopsis thaliana (62%), Portulaca oleracea (61%), Portulaca grandiflora (60%), and Zea mays (55%). The expression profile was studied in different plant tissues (stem, leaf, and root) under the abiotic stresses salt, drought, and cold. The results showed a 7-fold increased expression of GPGR5 in leaf tissue in salt stress and almost no induction of transcription in root and stem tissues in salt stress. This gene has a good expression pattern under cold stress compared to salt and drought. In future studies, GPGR5 may be used as a prognostic marker for the development of abiotic-stress-tolerant plants.
DOI
10.3906/biy-1504-30
Keywords
Gossypium arboreum, differential display PCR, RACE, proton gradient regulator 5, expression profiling
First Page
889
Last Page
898
Recommended Citation
SHAHID, M, JAMAL, A, AFTAB, B, MOHAMED, B. B, WATTOO, J, KIANI, M. S, RASHID, B, & HUSNAIN, T (2016). Identification, characterization, and expression profiling of salt-stress tolerant proton gradient regulator 5 (PGR5) in Gossypium arboreum. Turkish Journal of Biology 40 (4): 889-898. https://doi.org/10.3906/biy-1504-30
