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Turkish Journal of Biology

DOI

10.3906/biy-1506-66

Abstract

During the process of industrial production of biofuels and biochemicals from lignocellulosic biomass, large amounts of waste byproducts rich in xylose are generated, resulting in excessive wastage of natural resources and environmental pollution. In this work, xylose solution from corncob hydrolysate was utilized to produce 2,3-butanediol (2,3-BD), using the strain Enterobacter cloacae CICC 10011 to improve the utilization rate of hemicellulose and reduce environmental pollution. 2,3-BD fermentation conditions were subsequently developed. Xylose solution and (NH4)2HPO4, selected with the Plackett-Burman experiment, were determined as significant independent variables to conduct a response surface experiment. With the optimized medium, 50.02 g/L 2,3-BD production was obtained, which corresponded to 85.16% of the theoretical value. Furthermore, 81.4 g/L 2,3-BD production, 92.95 g/L the total production (2,3-BD + acetoin), and 0.72 g/(L h) productivity were obtained by fed-batch fermentation. Therefore, efficient production of 2,3-BD from corncob-derived xylose is important in the future development and expansion of biorefining technologies.

Keywords

Fermentation, cellulose, 2, 3-butanediol, bioreactors, Enterobacter cloacae, microbial growth

First Page

856

Last Page

865

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