Inducing osmotic stress leads to better genetic transformation efficiency in cotton (Gossypium hirsutum L.)

Authors

SURENDRA BARPETE
ALLAH BAKHSH
EMİNE ANAYOL
SANCAR FATİH ÖZCAN
Muhammet Cağri OĞUZ
ÖMER CEM KARAKOÇ
SEBAHATTİN ÖZCAN

DOI

10.3906/biy-1509-16

Abstract

The present study investigated the effect of different salts on cotton shoot regeneration and transformation efficiency. Two-day-old germinating embryos of a local cotton cultivar (SG-125) were pretreated with 50 mM each of NaCl, CaCl2, and KCl for 60 min. The embryo explants were transformed by cocultivation with Agrobacterium tumefaciens strain LBA 4404 harboring a binary plasmid pTF101.1 that carried the insecticidal gene (cry1Ac) under control of wound-inducible promoter (AoPR1) and bilanafos acetyl reductase (bar) gene for plant selection. The salt-pretreated embryos showed maximum response on regeneration MS medium containing 0.50 mg/L 6-benzylaminopurine (BAP) and 0.10 mg/L indole-3-butyric acid (IBA), also supplemented with 5 mg/L bialaphos for in vitro screening of the transformed plantlets. The primary transformants were further screened by molecular techniques for integration and expression of the introduced gene. Maximum transformation efficiency (1.10%) was noted on KCl-treated explants compared to nontreated (control) explants. In conclusion, pretreatment of explants with 50 mM KCl for 60 min induced positive effects and triggered shoot regeneration in primary cotton transformants.

Keywords

Cotton, in vitro, osmotic treatment, regeneration, transformation

First Page

826

Last Page

836

Recommended Citation

BARPETE, SURENDRA; BAKHSH, ALLAH; ANAYOL, EMİNE; ÖZCAN, SANCAR FATİH; OĞUZ, Muhammet Cağri; KARAKOÇ, ÖMER CEM; and ÖZCAN, SEBAHATTİN (2016) "Inducing osmotic stress leads to better genetic transformation efficiency in cotton (Gossypium hirsutum L.)," Turkish Journal of Biology: Vol. 40: No. 4, Article 12. https://doi.org/10.3906/biy-1509-16
Available at: https://journals.tubitak.gov.tr/biology/vol40/iss4/12