Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29(1-57) obtained from the wheat × Thinopyrum intermedium (wheatgrass) cross. This line is a high-protein line resistant to leaf rust, yellow rust, and powdery mildew under field conditions and has 42 chromosomes. GISH analysis using a total genomic DNA probe from Thinopyrum intermedium resulted in the identification of 14 chromosomes of wheatgrass origin. The sequential GISH patterns obtained using St-genomic and J-genomic DNA as probes and ABD-genomic DNA from wheat as a blocker led to a better determination of the genomic type of wheatgrass chromosomes. Rearrangements involving Thinopyrum chromosomes and the A, B, and D genomes of wheat were not observed. FISH using the repetitive DNA probes pSc119.2, Afa-family, and pTa71 allowed for identification of all the wheat chromosomes present.
GEORGIEVA, MARIYANA; KRUPPA, KLAUDIA; TYANKOVA, NEDYALKA; and LANG, MARTA MOLNAR
"Molecular cytogenetic identification of a novel hexaploid wheat-Thinopyrum intermedium partial amphiploid with high protein content,"
Turkish Journal of Biology: Vol. 40:
3, Article 4.
Available at: https://journals.tubitak.gov.tr/biology/vol40/iss3/4