Turkish Journal of Biology




In the present study, the effects of three ethylene inhibitors, silver nitrate (AgNO3), silver sulfate (Ag2SO4), and cobalt chloride (CoCl2), on the morphogenic and biochemical responses in CAB-6P and Gisela 6 cherry rootstocks were investigated. In both rootstocks, AgNO3 and Ag2SO4 promoted shoot regeneration while CoCl2 had such an effect only in Gisela 6. The rooting percentage of CAB-6P and Gisela 6 explants was diminished with AgNO3, Ag2SO4, and CoCl2. AgNO3 increased root number (40 μM) and root length (50 μM) in the CAB-6P rootstock while it had an inhibitory effect in Gisela 6. Ag2SO4 and CoCl2 enhanced root length of Gisela 6 explants whereas Ag2SO4 decreased root number and root length of CAB-6P explants. CoCl2 at 10 μM in both rootstocks augmented root number whereas 20 μM CoCl2 increased root length of CAB-6P microshoots. AgNO3, Ag2SO4, and CoCl2 decreased leaf chlorophyll and carbohydrate content in CAB-6P rootstock. On the other hand, in Gisela 6, 10 μM AgNO3 gave elevated chlorophyll levels and 20 μM AgNO3 or 10-50 μM CoCl2 raised leaf carbohydrate content. The three ethylene inhibitors led to elevated leaf proline levels in the CAB-6P rootstock, whereas in Gisela 6 the increase or decrease in leaf proline content was dependent on both type and concentration of ethylene inhibitor. An efficient micropropagation protocol was established for the CAB-6P and Gisela 6 cherry rootstocks using ethylene inhibitors.


Carbohydrates, cherry rootstocks, chlorophyll content, ethylene inhibitors, micropropagation, shoot multiplication, proline, rooting

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