Melatonin attenuates apoptosis and mitochondrial depolarization levels in hypoxic conditions of SH-SY5Y neuronal cells induced by cobalt chloride (CoCl2)

Authors

ABDÜLHADİ CİHANGİR UĞUZ
AHMİ ÖZ
BÜŞRA YILMAZ
SEDA ALTUNBAŞ
ÖMER ÇELİK

Abstract

Melatonin (MEL) and its metabolites serve as endogenous reactive oxygen species (ROS) scavengers and have a wide spectrum of antioxidant activity. Cobalt chloride is one of the commonly used hypoxia-mimetic agents, due to blocking the degradation of and triggering the accumulation of hypoxia-inducible factor-1 alpha (HIF-1α ) protein, which is very well known as a critical regulator of the cellular response against hypoxia. In the current study we aimed to determine the possible protective effects of melatonin on a cobalt chloride-induced hypoxia model of SH-SY5Y neuronal cells. Group I was the control group and SH-SY5Y cells were incubated in normal culture media without any chemical administration. In Group II, SH-SY5Y cells were incubated with 1 μM MEL for 24 h. In Group III cells were incubated with 200 μM cobalt chloride for 24 h. The last group, Group 4, was a combination group of cobalt chloride and MEL. Cells were preincubated with 1 μM for 12 h and then 200 μM cobalt chloride for 24 h. We performed the cell viability test (MTT) and checked the caspase-3 and -9 activities and the lipid peroxidation (LP), reduced glutathione (GSH), glutathione peroxidase (GSH-Px), mitochondrial depolarization, and intracellular ROS levels. We observed that cobalt chloride increased intracellular ROS, caspase-3 and -9, lipid peroxidation, and mitochondrial membrane depolarization values, while decreasing GSH levels and GSH-Px activity. However, GSH and GSH-Px values were increased by melatonin treatment although lipid peroxidation level, intracellular ROS production, and caspase-3 and -9 activities were decreased by the treatment. In conclusion, we observed that melatonin incubation protects neuronal cells against hypoxia-induced oxidative stress.

DOI

10.3906/biy-1505-100

Keywords

SH-SY5Y neuroblastoma cells, cobalt chloride, oxidative stress, melatonin, mitochondrial membrane depolarization

First Page

896

Last Page

903

Recommended Citation

UĞUZ, ABDÜLHADİ CİHANGİR; ÖZ, AHMİ; YILMAZ, BÜŞRA; ALTUNBAŞ, SEDA; and ÇELİK, ÖMER (2015) "Melatonin attenuates apoptosis and mitochondrial depolarization levels in hypoxic conditions of SH-SY5Y neuronal cells induced by cobalt chloride (CoCl2)," Turkish Journal of Biology: Vol. 39: No. 6, Article 11. https://doi.org/10.3906/biy-1505-100
Available at: https://journals.tubitak.gov.tr/biology/vol39/iss6/11