An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies

Authors

Arulvilee RAJASEGAR
Asyraf MANSOR
Ranjetta POOBATHY
Elayabalan SIVALINGAM
Uma Rani SINNIAH
SREERAMANAN SUBRAMANIAM

Abstract

Ascocenda Wangsa Gold is a new and fascinating orchid hybrid in the Malaysian flower industry. An efficient plant vitrification solution 2 (PVS2) cryopreservation technique was developed for protocorm-like bodies (PLBs) of Ascocenda Wangsa Gold orchid. Parameters assessed included the effect of PVS2 exposure periods, thawing duration, temperature, and culture conditions based on 2,3,5-triphenyltetrazolium chloride absorbance readings and regrowth rates. A regrowth rate of 33.3% was obtained after 2 months when the PLBs were dehydrated in PVS2 for 30 min. The growth rate was improved to 47% when thawing was conducted at 45 °C for 85 s. The highest growth rate (53.3%) was obtained when the PLBs were subjected to a 7-day dark treatment before being transferred to a 16-h/8-h light/dark photoperiod. Histological analyses were conducted to study the morphology of cryopreserved and noncryopreserved PLBs of Ascocenda Wangsa Gold. The vitrification protocol developed in this study is a feasible and safe method for strengthening the germplasm conservation of this orchid for commercial purposes.

DOI

10.3906/biy-1405-7

Keywords

Plant vitrification solution 2, liquid nitrogen, 2, 3, 5-triphenyltetrazolium chloride assay, Ascocenda Wangsa Gold, histology

First Page

202

Last Page

209

Recommended Citation

RAJASEGAR, Arulvilee; MANSOR, Asyraf; POOBATHY, Ranjetta; SIVALINGAM, Elayabalan; SINNIAH, Uma Rani; and SUBRAMANIAM, SREERAMANAN (2015) "An improved PVS2 cryopreservation technique for Ascocenda Wangsa Gold orchid using protocorm-like bodies," Turkish Journal of Biology: Vol. 39: No. 2, Article 3. https://doi.org/10.3906/biy-1405-7
Available at: https://journals.tubitak.gov.tr/biology/vol39/iss2/3