This study examines the influence of the polyamines putrescine and spermidine on the efficiency of androgenesis in anther cultures of 2 carrot cultivars, Kazan F_1 and Narbonne F_1, and the effect of putrescine on the process of plant regeneration from androgenetic embryos. In the Kazan F_1 variety, an increase in the number of obtained embryos was achieved using each of the 2 polyamines separately and in combination. In contrast, no beneficial effects of polyamines on the efficiency of androgenesis were observed in the Narbonne F_1 variety. Putrescine added to the regeneration medium increased the number of obtained plants. For embryos obtained on induction medium without putrescine, the best concentration was 160 mg putrescine in 1 L of regeneration medium, and for those obtained on the induction medium with putrescine the best concentration was 0.5 mg putrescine per 1 L. All of the resulting plants, both in the experimental and control combinations, had a doubled set of chromosomes. The vast majority of them were homozygous for both isoenzymes, glucose-6-phosphate isomerase and aspartate aminotransferase, and the distribution of homo- and heterozygous received combinations with the polyamines and in the control was very similar.
GÓRECKA, KRYSTYNA; KISZCZAK, WALDEMAR; KRZYZANOWSKA, DOROTA; KOWALSKA, URSZULA; and KAPUSCINSKA, AGATA
"Effect of polyamines on in vitro anther cultures of carrot (Daucus carota L.).,"
Turkish Journal of Biology: Vol. 38:
5, Article 6.
Available at: https://journals.tubitak.gov.tr/biology/vol38/iss5/6