Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)

Authors

HANDAN BALTA
ÖZGE KARAKAŞ METİN
FUNDA ŞENTÜRK AKFIRAT
FAHRİYE ERTUĞRUL
SEMRA HASANÇEBİ
YILDIZ AYDİN
KADİR AKAN
ZAFER MERT
MÜGE TÜRET
AHU ALTINKUT UNCUOĞLU

Abstract

An important application of molecular markers in plant systems involves improvement in the efficiency of conventional plant breeding by carrying out indirect selection through molecular markers linked to the traits of interest. AFLP analysis was used to identify molecular markers associated with yellow rust resistance in wheat (Triticum aestivum L.) in this study. DNA isolated from the selected yellow rust tolerant and susceptible F_2 individuals derived from a cross between Izgi2001 (resistant) and ES14 (susceptible) at seedling and adult stage was used for bulked segregant analysis combined with AFLP. From the screening of 34 PstI/MseI AFLP primer combinations, the AFLP marker P-GAC/M-ACG (133 bp) was identified and presented in the resistant parent and resistant F_2 individuals but not in the susceptible ones. Future research will obtain more adjacent sequences associated with the polymorphic M-ACG/P-GAC_{133bp} marker by the PCR walking method to design SCAR markers for wheat breeding programs.

DOI

10.3906/biy-1311-8

Keywords

AFLP, bread wheat, disease resistance, SCAR, yellow rust

First Page

371

Last Page

379

Recommended Citation

BALTA, HANDAN; METİN, ÖZGE KARAKAŞ; AKFIRAT, FUNDA ŞENTÜRK; ERTUĞRUL, FAHRİYE; HASANÇEBİ, SEMRA; AYDİN, YILDIZ; AKAN, KADİR; MERT, ZAFER; TÜRET, MÜGE; and UNCUOĞLU, AHU ALTINKUT (2014) "Identification of an AFLP marker linked with yellow rust resistance in wheat (Triticum aestivum L.)," Turkish Journal of Biology: Vol. 38: No. 3, Article 8. https://doi.org/10.3906/biy-1311-8
Available at: https://journals.tubitak.gov.tr/biology/vol38/iss3/8