Temperature-induced stress response in Lymantria dispar neurosecretory neurons

Authors

LARISA ILIJIN
MILENA VLAHOVIC
VESNA PERIC MATARUGA
IVANA KMETIC
ANJA GAVRILOVIC
DRAGANA MATIC
MARIJA MRDAKOVIC

DOI

10.3906/biy-1306-75

Abstract

The release of neurosecretory material from A2 neurosecretory neurons (NSNs) was stimulated in Lymantria dispar fourth instar caterpillars exposed to a temperature of 35 °C for 1, 12, and 24 h, as well as those allowed recover after exposure (12 h at 35 °C, then 12 h at 23 °C). The levels of 2 protein forms with the same molecular mass as bombyxin (3-4 and 4-5 kDa) increased with prolonged exposure to 35 °C. The second band was present only in the groups exposed to this stressor. There was intensified synthetic activity and a low level of secretion in L2' NSNs after exposure to 35 °C. We previously found these NSNs to be immunopositive for prothoracicotropic neurohormone. After this stress, densitometric analysis revealed a decreased amount of the 11-12 kDa isoform (present in the control group). The new isoform (13-15 kDa), expressed after exposure of the insects to a high temperature, increased in amount with prolonged exposure and after recovery at 23 °C. Short-term exposure of caterpillars to high temperatures (35 °C) is a stressor and activates carbohydrate metabolism, while PTTH immunopositive NSNs are secretory-inactive during acute thermal stress regimes.

Keywords

High temperature, gypsy moth, medial and lateral neurosecretory neurons

First Page

157

Last Page

167

Recommended Citation

ILIJIN, LARISA; VLAHOVIC, MILENA; MATARUGA, VESNA PERIC; KMETIC, IVANA; GAVRILOVIC, ANJA; MATIC, DRAGANA; and MRDAKOVIC, MARIJA (2014) "Temperature-induced stress response in Lymantria dispar neurosecretory neurons," Turkish Journal of Biology: Vol. 38: No. 2, Article 1. https://doi.org/10.3906/biy-1306-75
Available at: https://journals.tubitak.gov.tr/biology/vol38/iss2/1