Turkish Journal of Biology
Abstract
The release of neurosecretory material from A2 neurosecretory neurons (NSNs) was stimulated in Lymantria dispar fourth instar caterpillars exposed to a temperature of 35 °C for 1, 12, and 24 h, as well as those allowed recover after exposure (12 h at 35 °C, then 12 h at 23 °C). The levels of 2 protein forms with the same molecular mass as bombyxin (3-4 and 4-5 kDa) increased with prolonged exposure to 35 °C. The second band was present only in the groups exposed to this stressor. There was intensified synthetic activity and a low level of secretion in L2' NSNs after exposure to 35 °C. We previously found these NSNs to be immunopositive for prothoracicotropic neurohormone. After this stress, densitometric analysis revealed a decreased amount of the 11-12 kDa isoform (present in the control group). The new isoform (13-15 kDa), expressed after exposure of the insects to a high temperature, increased in amount with prolonged exposure and after recovery at 23 °C. Short-term exposure of caterpillars to high temperatures (35 °C) is a stressor and activates carbohydrate metabolism, while PTTH immunopositive NSNs are secretory-inactive during acute thermal stress regimes.
DOI
10.3906/biy-1306-75
Keywords
High temperature, gypsy moth, medial and lateral neurosecretory neurons
First Page
157
Last Page
167
Recommended Citation
ILIJIN, L, VLAHOVIC, M, MATARUGA, V. P, KMETIC, I, GAVRILOVIC, A, MATIC, D, & MRDAKOVIC, M (2014). Temperature-induced stress response in Lymantria dispar neurosecretory neurons. Turkish Journal of Biology 38 (2): 157-167. https://doi.org/10.3906/biy-1306-75
