In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Authors

SATI UZUN
İSKENDER PARMAKSIZ
SERKAN URANBEY
SEMRA MİRİCİ
ERCÜMENT OSMAN SARIHAN
ARİF İPEK
MEHMET DEMİR KAYA
BİLAL GÜRBÜZ
NEŞET ARSLAN
CENGİZ SANCAK
KHALID MAHMOOD kHAWAR
SEBAHATTİN ÖZCAN

Abstract

An efficient in vitro bulblet production procedure from immature zygotic embryos of endemic and endangered Muscari muscarimi Medik. was described in the current study. Zygotic embryos were first isolated from immature seeds and cultured on different nutrient media compositions supplemented with various combinations of \alpha-naphthalene acetic acid (NAA), picloram, dicamba, 6-benzylaminopurine (BAP), and thidiazuron (TDZ). The best bulblet regeneration (59 bulblets per explant) was achieved in Murashige and Skoog (MS) medium containing 4 mg/L BAP and 0.5 mg/L NAA after 1 year of culture initiation. Regenerated bulblets were then transferred into MS medium without plant growth regulators for rooting. Bulblets produced well-developed root systems and increased their size on this medium after 2 months. All rooted bulblets were successfully transplanted into a potting mixture and acclimatized to ambient conditions.

DOI

10.3906/biy-1307-52

Keywords

Muscari muscarimi Medik., immature embryo, tissue culture

First Page

83

Last Page

88

Recommended Citation

UZUN, SATI; PARMAKSIZ, İSKENDER; URANBEY, SERKAN; MİRİCİ, SEMRA; SARIHAN, ERCÜMENT OSMAN; İPEK, ARİF; KAYA, MEHMET DEMİR; GÜRBÜZ, BİLAL; ARSLAN, NEŞET; SANCAK, CENGİZ; kHAWAR, KHALID MAHMOOD; and ÖZCAN, SEBAHATTİN (2014) "In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.," Turkish Journal of Biology: Vol. 38: No. 1, Article 9. https://doi.org/10.3906/biy-1307-52
Available at: https://journals.tubitak.gov.tr/biology/vol38/iss1/9