Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew

Authors

Zohreh JALALPOUR
LEILA SHABANI
Ladan AFGHANI
Majid SHARIFI-TEHRANI
SAYED ASADOLLAH AMINI

Abstract

Cell suspension cultures of Taxus baccata were treated with 2 elicitor compounds, methyl jasmonate (MeJA) and squalestatin (S), individually and in combination for 7 days to determine if they mediated the enhancement of biosynthesis of endogenous jasmonate through induction of lipoxygenase (LOX) activity. Total phenolic compounds, total flavonoids, total antioxidants, phenylalanine ammonia-lyase (PAL), polyphenol oxidase (PPO), and LOX activities in 5-month-old yew cell cultures were studied. Our results showed that MeJA and S could stimulate production of phenol derivatives in cell suspension cultures of T. baccata. In parallel to the induction of phenolic production in elicited cells, results showed that activities of PAL and PPO enzymes and total antioxidants significantly increased in Taxus cells in response to MeJA and S. Maximal activities of lipoxygenase were obtained 48 h after treatment with MeJA (100 µM), S (0.1 µM), and the combination of the 2 elicitors. Results showed that MeJA and S are effective elicitors for increasing phenolic production in Taxus cell suspension cultures, likely through increasing LOX activity followed by an increase in endogenous jasmonate.

DOI

10.3906/biy-1306-91

Keywords

Antioxidant, lipoxygenase, phenylalanine ammonia-lyase, squalestatin, Taxus

First Page

76

Last Page

82

Recommended Citation

JALALPOUR, Zohreh; SHABANI, LEILA; AFGHANI, Ladan; SHARIFI-TEHRANI, Majid; and AMINI, SAYED ASADOLLAH (2014) "Stimulatory effect of methyl jasmonate and squalestatin on phenolic metabolism through induction of LOX activity in cell suspension culture of yew," Turkish Journal of Biology: Vol. 38: No. 1, Article 8. https://doi.org/10.3906/biy-1306-91
Available at: https://journals.tubitak.gov.tr/biology/vol38/iss1/8