Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers

Authors

LILIANA JARDA
ANCA BUTIUC KEUL
MARIA HOHN
ANDRZEJ PEDRYC
VICTORIA CRISTEA

Abstract

Ex situ conservation of Dianthus giganteus subsp. banaticus was obtained by in vitro culture on different culture media supplemented with phytohormones. Assessment of somaclonal variability over a period of preservation by in vitro culture is required prior to introduction and acclimatization in natural habitats. In this study we used 2 types of molecular markers, ISSR and multilocus SSR markers, which allow comparison of different alleles, to assess the somaclonal variability of endemic taxa Dianthus giganteus subsp. banaticus after a period of 24 months of preservation by in vitro culture. The assessment of somaclonal variability of D. giganteus subsp. banaticus shows some changes in the absence/presence of certain markers in some individuals.

DOI

10.3906/biy-1303-20

Keywords

ISSR, SSR markers, Dianthus giganteus subsp. banaticus, ex situ conservation, in vitro culture, somaclonal variability

First Page

21

Last Page

30

Recommended Citation

JARDA, LILIANA; KEUL, ANCA BUTIUC; HOHN, MARIA; PEDRYC, ANDRZEJ; and CRISTEA, VICTORIA (2014) "Ex situ conservation of Dianthus giganteus d’Urv. subsp. banaticus (Heuff.) Tutin by in vitro culture and assessment of somaclonal variability by molecular markers," Turkish Journal of Biology: Vol. 38: No. 1, Article 3. https://doi.org/10.3906/biy-1303-20
Available at: https://journals.tubitak.gov.tr/biology/vol38/iss1/3