A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation

Authors

ERKAN MOZİOĞLU
MÜSLÜM AKGÖZ
CANDAN TAMERLER
ZÜHTÜ TANIL KOCAGÖZ

Abstract

A high-quality and low-cost genomic DNA isolation method is needed for use in microbiology laboratories. In this study, we developed a new modified guanidinium isothiocyanate method to isolate DNA from Escherichia coli, Salmonella enterica subsp. enterica serotype Typhimurium, and Staphylococcus aureus and compared it with 4 other DNA isolation methods. The results show that the modified guanidinium isothiocyanate method developed in our laboratory is simple, fast, and inexpensive and yields DNA whose quality and quantity are similar to that of 2 commercial extraction kits but far better than 2 conventional DNA extraction methods used for comparison.

DOI

10.3906/biy-1308-7

Keywords

Bacteria, genomic DNA isolation, guanidinium isothiocyanate

First Page

125

Last Page

129

Recommended Citation

MOZİOĞLU, ERKAN; AKGÖZ, MÜSLÜM; TAMERLER, CANDAN; and KOCAGÖZ, ZÜHTÜ TANIL (2014) "A simple guanidinium isothiocyanate method for bacterial genomic DNA isolation," Turkish Journal of Biology: Vol. 38: No. 1, Article 14. https://doi.org/10.3906/biy-1308-7
Available at: https://journals.tubitak.gov.tr/biology/vol38/iss1/14