A comparative study of EGF effects on in vitro bovine embryo development in monoculture and sequential media

Authors

AZADEH ZAHMATKESH
MAHMOOD AMIRI ROUDBAR
MORTEZA DALIRI JOUPARI

Abstract

Different culture system conditions can affect cleavage, zygote genome activation, embryo development, and viability. The purpose of the present study was to compare a monoculture synthetic oviductal fluid medium with modified sequential G1/G2 media and also to investigate the effect of epidermal growth factor (EGF) on bovine embryo development. Oocytes were aspirated from 2- to 6-mm ovarian follicles and matured in maturation media. After in vitro fertilization of matured oocytes, presumptive zygotes were cultured in test media with or without additional exogenous EGF. Results indicated that EGF significantly increased blastocyst formation but not cleavage rate in both media. There was no significant difference between the sequential and the monoculture media.

DOI

10.3906/biy-1302-74

Keywords

Blastocyst, cleavage, culture system, epidermal growth factor

First Page

670

Last Page

674

Recommended Citation

ZAHMATKESH, AZADEH; ROUDBAR, MAHMOOD AMIRI; and JOUPARI, MORTEZA DALIRI (2013) "A comparative study of EGF effects on in vitro bovine embryo development in monoculture and sequential media," Turkish Journal of Biology: Vol. 37: No. 6, Article 5. https://doi.org/10.3906/biy-1302-74
Available at: https://journals.tubitak.gov.tr/biology/vol37/iss6/5