Induction of salt-tolerant potato (Solanum tuberosum L.) mutants with gamma irradiation and characterization of genetic variations via RAPD-PCR analysis

Authors

ORKUN YAYCILI
SEMA ALİKAMANOĞLU

Abstract

Salt-tolerant mutants of potato (Solanum tuberosum L. 'Marfona') were obtained via gamma irradiation. Node explants of the Marfona potato plant were treated with various dosages of gamma irradiation, and the M_1V_2 and M_1V_3 clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 50, 100, and 125 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using RAPD-PCR method, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants were genetically 27.5% different from the control plants. The greatest difference encountered between the control and mutants was 47%, which was detected in mutant plants produced by 20 or 30 Gy gamma irradiation and regenerated in selection medium containing 100 mM NaCl.

DOI

10.3906/biy-1110-14

Keywords

Gamma radiation, in vitro mutagenesis, mutation breeding, potato, RAPD-PCR, salt stress

First Page

405

Last Page

412

Recommended Citation

YAYCILI, ORKUN and ALİKAMANOĞLU, SEMA (2012) "Induction of salt-tolerant potato (Solanum tuberosum L.) mutants with gamma irradiation and characterization of genetic variations via RAPD-PCR analysis," Turkish Journal of Biology: Vol. 36: No. 4, Article 5. https://doi.org/10.3906/biy-1110-14
Available at: https://journals.tubitak.gov.tr/biology/vol36/iss4/5