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Turkish Journal of Biology

Abstract

İzmir Çamaltı Saltern is the biggest seawater-based saltern in Turkey. To date, it has not been investigated extensively for the existence of poly-3-hydroxybutyrate (PHB)-producing species. In this study, 14 extremely halophilic archaea were isolated, purified, and screened for PHB production. One strain was then selected as the best PHB producer, further cultivated in different PHB media, and compared with a positive control, Haloferax mediterranei ATCC 33500. PHB was extracted from cells and measured with a spectrophotometer, and then the amount of PHB was measured through comparison with standard PHB. The detected high PHB yield was 6.53% of the dry cell weight in the PHB medium supplemented with acetate. Partial 16S rRNA gene sequence analysis showed 99% similarity to Haloferax alexandrinus strain TM^T; therefore, the strain was named Haloferax sp. MA10. Haloferax sp. MA10 and Haloferax alexandrinus TM^T have some differences in phenotypic and biochemical properties. With this study, the discovery of PHB-producing extreme halophilic archaeon Haloferax sp. MA10 at İzmir's Çamaltı Saltern is reported for the first time.

DOI

10.3906/biy-1102-16

Keywords

Extremely halophilic archaeon, poly-3-hydroxybutyrate (PHB), PHB extraction and quantification, Haloferax sp. MA10, 16S rRNA gene

First Page

303

Last Page

312

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