The present study investigates changes in the inorganic ions, proline, and endogenous abscisic acid (ABA) contents of the apoplastic and symplastic compartments of leaves from drought-tolerant (Yakutiye) and drought-sensitive (Zulbiye) cultivars of the common bean (Phaseolus vulgaris L.). Drought stress caused a decrease in leaf water potential and stomatal conductance in both cultivars. Concentrations of proline in the drought-tolerant and drought-sensitive cultivars increased in response to drought stress in both compartments. The symplastic K^+ concentration decreased in both cultivars. However, the opposite trend was observed concerning K^+ concentrations in the apoplastic areas. While the symplastic Na^+ concentrations significantly decreased in the drought-tolerant cultivar, the apoplastic Na^+ concentrations increased during drought stress. However, Na^+ concentrations did not significantly change in either of the compartments in the drought-sensitive cultivar. The Ca^2^+ concentrations in the sensitive cultivar significantly decreased in both compartments during drought stress. In the tolerant cultivar, the Ca^2^+ concentration significantly increased in the symplast but decreased in the apoplast. Cl^- concentrations in the tolerant cultivar did not significantly change in either compartment. In the sensitive cultivar, the Cl^- concentration increased in the apoplastic area but decreased in the symplastic area. In addition, while the symplastic sap of the leaves exhibited a constant pH value, it diminished in the apoplast during drought stress. Symplastic and apoplastic ABA concentrations significantly increased in both cultivars. It might be said that inorganic ions (especially Na^+, K^+, and Ca^2^+) and ABA concentrations changed between the apoplastic and symplastic spaces to contribute to osmotic adjustment under drought stress. In addition, the drought-tolerant cultivar showed a much higher capacity to maintain osmotic adjustment between the symplast and the apoplast.
Apoplast, symplast, drought stress, tolerance, inorganic ions, abscisic acid
GÜLER, NESLİHAN SARUHAN; SAĞLAM, AYKUT; DEMİRALAY, MEHMET; and KADIOĞLU, ASİM
"Apoplastic and symplastic solute concentrations contribute to osmotic adjustment in bean genotypes during drought stress,"
Turkish Journal of Biology: Vol. 36:
2, Article 3.
Available at: https://journals.tubitak.gov.tr/biology/vol36/iss2/3