Turkish Journal of Biology
Abstract
Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics use in livestock production. The present study investigated the pattern and mechanisms of resistance in 21 bacteria species isolated from a river used as a waste-dump by a poultry farm in Ogbomoso, southwestern Nigeria. Bacteria were isolated on nutrient agar supplemented with 50 µg/mL of tetracycline, their identities were determined by PCR amplification, and sequencing of their 16s rRNA genes and their susceptibility to 6 antimicrobials determined by broth dilution. The molecular basis of resistance among the isolates was also investigated by the PCR amplification of class 1 and 2 integrons, tetA, tetB, tetC, tetD, tetE, tetG, tetL, tetW, tetM, tet37, bla_{TEM}, aadA1, strA, strB, sul2, sul3 and a 550bp gene fragment encoding a multidrug resistance efflux transporter of the resistance nodulation division (RND) family. All the isolates were resistant to the tested antimicrobials with MIC ranging from
DOI
10.3906/biy-1010-145
Keywords
16s rRNA, resistance genes, RND transporter, integrons
First Page
37
Last Page
45
Recommended Citation
ADELOWO, OLAWALE OLUFEMI and FAGADE, OBASOLA EZEKIEL
(2012)
"Phylogenetic characterization, antimicrobial susceptibilities, and mechanisms of resistance in bacteria isolates from a poultry waste-polluted river, southwestern Nigeria,"
Turkish Journal of Biology: Vol. 36:
No.
1, Article 5.
https://doi.org/10.3906/biy-1010-145
Available at:
https://journals.tubitak.gov.tr/biology/vol36/iss1/5
