Turkish Journal of Biology
Abstract
This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and at 40 °C for 60 min. Ca^{2+} and Mn^{2+} enhanced lipase activity but it was determined that other metallic ions did not affect the enzyme activity. The K_{m} and V_{max} values of the crude enzyme for p-nitrophenyl butyrate hydrolysis were found to be 7.15 mM and 7.067 mM/min, respectively.
DOI
10.3906/biy-1004-107
Keywords
Trichoderma harzianum, extracellular lipase, enzyme characterization
First Page
543
Last Page
550
Recommended Citation
ÜLKER, S, ÖZEL, A, ÇOLAK, A, & KARAOĞLU, Ş. A (2011). Isolation, production, and characterization of an extracellular lipase from Trichoderma harzianum isolated from soil. Turkish Journal of Biology 35 (5): 543-550. https://doi.org/10.3906/biy-1004-107
