Turkish Journal of Biology
DOI
10.3906/biy-0912-25
Abstract
A gene encoding \beta-(1,3-1,4)-glucanase (licA) was amplified from Orpinomyces sp. GMLF18 and expressed in Escherichia coli. The DNA sequence of licA showed that the gene was 707 bp and encoded a protein with a molecular mass of 26 kDa that belongs to family glycosyl hydrolase 16. The main LicA activity was observed to be cell-associated for the licA containing transformant E. coli, and the enzyme expressed in E. coli showed the highest activity at pH 5.0-6.0 and at temperatures of 40-50 °C. The enzyme was found to be stable at 40 °C; however, 12% of LicA activity was lost at 50 °C in 20 min. The licA was then introduced into the facultative anaerobic bacterium Lactococcus lactis subsp. cremoris MG1363 by a stable recombinant plasmid, pIL253. Although the enzymatic activity was lower than that in E. coli, the gene encoding the fungal originated lichenase was successfully expressed in L. lactis.
Keywords
Orpinomyces, \beta-(1, 3-1, 4)-glucanase, licA, Lactococcus lactis, lichenan
First Page
405
Last Page
414
Recommended Citation
ÇÖMLEKCİOĞLU, UĞUR; ÖZKÖSE, EMİN; AKYOL, İSMAİL; YAZDIÇ, FERİT CAN; and EKİNCİ, MEHMET SAİT
(2011)
"Expression of β-(1,3-1,4)-glucanase gene of Orpinomyces sp. GMLF18 in Escherichia coli EC1000 and Lactococcus lactis subsp. cremoris MG1363,"
Turkish Journal of Biology: Vol. 35:
No.
4, Article 3.
https://doi.org/10.3906/biy-0912-25
Available at:
https://journals.tubitak.gov.tr/biology/vol35/iss4/3