•  
  •  
 

Turkish Journal of Biology

DOI

10.3906/biy-0807-30

Abstract

This study investigated the developmental toxicity of a plant growth regulator (a type of pesticide) using the frog embryo teratogenesis assay-Xenopus (FETAX). Xenopus laevis embryos were exposed to 11 different concentrations of gibberellic acid (GA_3), from stage 8 to 11, for 96 h under static renewal test conditions. The median lethal concentration (LC_{50}), malformation (EC_{50}), non-observed adverse effect concentration (NOAEC), and lowest observed adverse effect concentration (LOAEC) were calculated. The corresponding LC_{50} and EC_{50} values determined for GA3 exposure were 1117.5 mg/L and 658 mg/L, respectively. The TI (LC50/EC50) value calculated for GA_3 was 1.69. Different anomalies occurred in the embryos, depending on the GA_3 concentration. Based on these results, we conclude that GA_3 is toxic and teratogenic to Xenopus laevis embryos. Moreover, our results confirm that the FETAX assay can be a useful pretest for integrated biological hazard assessment of chemical agents used in agriculture.

Keywords

Gibberellic acid, FETAX, Teratogenicity, Toxicity, Xenopus laevis

First Page

181

Last Page

188

Download

Included in

Biology Commons

Share

COinS