Hypoxia Induced Changes in the Lipid Peroxidation, Membrane Permeability, Reactive Oxygen Species Generation, and Antioxidative Response Systems in Zea mays Leaves

Authors

RASHID JAMEI
REZA HEIDARI
JALIL KHARA
SAMAD ZARE

Abstract

The effects of hypoxia on the activities of some enzymes of antioxidative, non- enzymatic scavenging system, membrane permeability, lipid peroxidation, and some reactive oxygen species (ROS) in leaves of Zea mays were investigated. Samples were taken 48, 96, 144, and 192 h after the start of hypoxia treatment. A 192 h hypoxia treatment resulted in a significant rise in membrane permeability, lipid peroxidation (malondialdehyde level), and the production of hydrogen peroxide (H_2O_2) and superoxide (O_2^-) in maize leaves. A short duration of hypoxia enhanced the activity of superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), and glutathione reductase (GR; EC 1.6.4.2), while further hypoxia significantly decreased the enzyme activity but increased the content of reduced glutathione (GSH) and ascorbic acid (AsA). It was observed that the reduction in SOD activity was greater than that in GR and APX (H_2O_2 scavengers). Our results showed that O_2^- induced membrane damage and lipid peroxidation, and that excessive accumulation of O_2^- is due to the reduced activity of SOD under hypoxia.

DOI

10.3906/biy-0807-14

Keywords

Antioxidant enzymes, ascorbate, glutathione, hypoxia, malondialdehyde, membrane permeability, reactive oxygen species, Zea mays

First Page

45

Last Page

52

Recommended Citation

JAMEI, RASHID; HEIDARI, REZA; KHARA, JALIL; and ZARE, SAMAD (2009) "Hypoxia Induced Changes in the Lipid Peroxidation, Membrane Permeability, Reactive Oxygen Species Generation, and Antioxidative Response Systems in Zea mays Leaves," Turkish Journal of Biology: Vol. 33: No. 1, Article 7. https://doi.org/10.3906/biy-0807-14
Available at: https://journals.tubitak.gov.tr/biology/vol33/iss1/7