The present work examined the in vitro antimicrobial and antioxidant activities of the essential oil and methanol extract from Achillea biebersteinii Afan. (Asteraceae). The essential oil exhibited antimicrobial activity against 8 bacteria, 14 fungi and the yeast C. albicans, whereas methanolic extract remained inactive. The antioxidative capacity of the samples was evaluated by using DPPH (2,2-diphenyl-1-picrylhydrazyl) and b-carotene/linoleic acid assays. In both assays, the extract showed better antioxidative capacity than the oil. The extract reduced the stable free radical DPPH with lower IC50 value (89.90 µg/ml) than the oil (8900 µg/ml). In the b-carotene/linoleic acid assay, the samples were not effectively able to inhibit the linoleic acid oxidation, exhibiting only 22.7% (the extract) and 16% (the essential oil) inhibitions at 2 mg/ml, far below than that of BHT (97.0%). Total phenolic constituent of the extract was 51 µg/mg (5.1%, w/w) as gallic acid equivalent. GC-MS analysis of the essential oil resulted in the identification of 64 components representing 92.24% of the oil. Piperitone, camphor and 1,8-cineole (eucalyptol) were the main constituents.
Achillea biebersteinii, Antimicrobial activity, Antioxidant activity, GC-MS analysis, piperitone, camphor, eucalyptol
BARIŞ, ÖZLEM; GÜLLÜCE, MEDİNE; ŞAHİN, FİKRETTİN; ÖZER, HAKAN; KILIÇ, HAMDULLAH; ÖZKAN, HAKAN; SÖKMEN, MÜNEVVER; and ÖZBEK, TÜLİN (2006) "Biological Activities of the Essential Oil and Methanol Extract of Achillea biebersteinii Afan. (Asteraceae)," Turkish Journal of Biology: Vol. 30: No. 2, Article 2. Available at: https://journals.tubitak.gov.tr/biology/vol30/iss2/2