Effects of Carbon Sources and Various Chemicals on the Production of a Novel Amylase from a Thermophilic Bacillus sp. K-12

Authors

ÖZLEM EREN KIRAN
UĞUR ÇÖMLEKÇİOĞLU
BURHAN ARIKAN

Abstract

The amylase producer thermophilic Bacillus sp. K-12 was isolated from soil samples from Zeytinli hot spring in Kahramanmaraş. Enzyme synthesis occurred at 20-55 ºC with an optimum of 42 ºC. There was a slight variation in amylase synthesis within the pH range 4.5-10.5. Effects of various carbon sources and chemicals on a-amylase production were examined and maximum a-amylase production was obtained in a medium containing 1% starch in 60 h. MnSO_4, ZnSO_4 and EDTA inhibited a-amylase production of Bacillus sp. K-12.

DOI

-

Keywords

Thermostable, Amylase, Bacillus

First Page

99

Last Page

103

Recommended Citation

KIRAN, ÖZLEM EREN; ÇÖMLEKÇİOĞLU, UĞUR; and ARIKAN, BURHAN (2005) "Effects of Carbon Sources and Various Chemicals on the Production of a Novel Amylase from a Thermophilic Bacillus sp. K-12," Turkish Journal of Biology: Vol. 29: No. 2, Article 3. Available at: https://journals.tubitak.gov.tr/biology/vol29/iss2/3