Adventitious Shoot Organogenesis and Plant Regeneration from Cotyledons of Diploid Diyarbakır Watermelon (Citrullus lanatus cv. "Sürme")

Authors

VEDAT PİRİNÇ
AHMET ONAY
FİLİZ ADIYAMAN
ÇİĞDEM IŞIKALAN
ENGİN TİLKAT
DAVUT BAŞARAN

Abstract

Adventitious shoots were obtained from the diploid Diyarbakır watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per explant were approximately more than 50% higher when 0.5 mg/l BA was used compared to the most effective kinetin concentration (1 mg/l). Plants were obtained by transferring 1 to 2 cm shoots to MS medium supplemented with 1-4 mg/l naphthalene acetic acid. Most of the regenerated plants (more than 50%) were successfully transplanted to the field. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbakır watermelon for use in breeding lines.

DOI

-

Keywords

Regeneration, Tissue culture, Watermelon

First Page

101

Last Page

105

Recommended Citation

PİRİNÇ, VEDAT; ONAY, AHMET; ADIYAMAN, FİLİZ; IŞIKALAN, ÇİĞDEM; TİLKAT, ENGİN; and BAŞARAN, DAVUT (2003) "Adventitious Shoot Organogenesis and Plant Regeneration from Cotyledons of Diploid Diyarbakır Watermelon (Citrullus lanatus cv. "Sürme")," Turkish Journal of Biology: Vol. 27: No. 2, Article 7. Available at: https://journals.tubitak.gov.tr/biology/vol27/iss2/7