Turkish Journal of Biology




The role of phospholipase D (EC was studied in the changing of phospholipid content during cotton seed (Gossipium hirsutum 108-G) maturation. To define phospholipase D activity cotton seed homogenates were incubated in a water-methanol environment at 37 °C for various time intervals (15, 30, 60, 120, and 300 min). Enzyme activity was judged from the formation of hydrolysis products - phosphatidic acid and phosphatidylmethanol. The phospholipid content was defined by using thin-layer chromatography with a follow up analysis of phosphorus quantity in phospholipid fractions. It was shown that phospholipase D displayed two functions during cotton seed maturation: transferase and hydrolase. Hydrolase function could be defined from the increase of the product (phosphatidic acid) concentration during the complete maturation of the seed. We noticed that the concentration increased. Transferase activity could be defined through the increase of the phosphatidylmetanol concentration during the incubation of phospholipids in a water-methanol environment. The experimental data showed that phospholipase D plays an important role in phospholipid metabolism.


Phospholipase D, Phospholipids, Cotton seeds.

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