Turkish Journal of Biology
Abstract
In this study, it was aimed to increase the frequency of shoots, which would be nominated to be transgenic after Agrobacterium inoculation, through expanding the wounding area by peeling the epidermis layer of hypocotyl explants of flax plants. Callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish were recorded as 0.8 g, 40%, 4.24 and 16.96, respectively in non-peeled explants. On the other hand, when the epidermis layer was peeled, callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish increased to 1.35 g, 58.75%, 8.15 and 47.88, respectively.
DOI
-
Keywords
Flax, injured area, callus-based shoot regeneration
First Page
95
Last Page
98
Recommended Citation
YILDIZ, MUSTAFA and ER, CELAL (2002) "Increasing the Injured Area on Hypocotyl Explants of Flax (Linum usitatissimum L.) Leads to High Frequency Callus-Based Shoot Regeneration," Turkish Journal of Biology: Vol. 26: No. 2, Article 8. Available at: https://journals.tubitak.gov.tr/biology/vol26/iss2/8
