In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxonomic tool for bacteria belonging to the genus Nocardia. A total of 21 Nocardia strains were clearly distinguished using a combination of EcoRV gene restriction endonuclease patterns together with a digoxigenin-labelled Streptomyces violaceoruber TK21 rDNA probe. Five distinct ribotypes were identified, and each ribotype contained 4 to 8 restriction fragments ranging in size from 1.2 to 20.7 kb. The evaluation of the results showed that N. brasiliensis, N. farcinica, N. otitidiscaviarum and N. seriolea are taxonomically well-established species, except N. pseudobrasiliensis. Therefore, it can be concluded that ribotyping technique provide a useful information for the classification of clinically significant Nocardia.
Nocardia, Ribotyping, Taxonomy
IŞIK, KAMİL; ŞAHİN, NEVZAT; KARİPTAŞ, ERGİN; and GOOFELLOW, MICHAEL (2002) "Typing of Some Clinically Significant Nocardia Strains Using a Digoxigenin-Labelled rDNA Gene Probe," Turkish Journal of Biology: Vol. 26: No. 1, Article 1. Available at: https://journals.tubitak.gov.tr/biology/vol26/iss1/1