Availability of Use of Total Extracellular Proteins in SDS-PAGE for Typing Staphylococcus aureus and Coagulase-Negative Staphylococci

Authors

SEFA CAN SAÇILIK
ÖZLEM OSMANAĞAOĞLU
A. HAKKI SAYAR
CUMHUR ÇÖKMÜŞ

DOI

-

Abstract

A total of nineteen Staphylococcus references, nine of which are Staphylococcus aureus and ten coagulase-negative Staphylococci (CNS), were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Whole-cell protein (WCP) profiles obtained by SDS-PAGE were compared with banding patterns produced by SDS-PAGE of total extracellular proteins (TEP). No report has yet been issued which explains Staphylococcus typing by SDS-PAGE of extracellular proteins. SDS-PAGE of whole-cell extracts did not usefully distinguish different isolates of Staphylococci. Our preliminary results suggest that SDS-PAGE of total extracellular proteins provides additional criteria and characteristic banding patterns for the study of the epidemiology and characterization of Staphylococci. SDS-PAGE of extracellular proteins provided a rapid, reproducible and discriminative method for characterization of Staphylococci which is easy to perform and interpret.

Keywords

Staphylococcus aureus, coagulase-negative staphylococci (CNS), protein profiles, typing.

First Page

145

Last Page

151

Recommended Citation

SAÇILIK, SEFA CAN; OSMANAĞAOĞLU, ÖZLEM; SAYAR, A. HAKKI; and ÇÖKMÜŞ, CUMHUR (2001) "Availability of Use of Total Extracellular Proteins in SDS-PAGE for Typing Staphylococcus aureus and Coagulase-Negative Staphylococci," Turkish Journal of Biology: Vol. 25: No. 2, Article 5. Available at: https://journals.tubitak.gov.tr/biology/vol25/iss2/5