Turkish Journal of Biology
Abstract
A total of nineteen Staphylococcus references, nine of which are Staphylococcus aureus and ten coagulase-negative Staphylococci (CNS), were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Whole-cell protein (WCP) profiles obtained by SDS-PAGE were compared with banding patterns produced by SDS-PAGE of total extracellular proteins (TEP). No report has yet been issued which explains Staphylococcus typing by SDS-PAGE of extracellular proteins. SDS-PAGE of whole-cell extracts did not usefully distinguish different isolates of Staphylococci. Our preliminary results suggest that SDS-PAGE of total extracellular proteins provides additional criteria and characteristic banding patterns for the study of the epidemiology and characterization of Staphylococci. SDS-PAGE of extracellular proteins provided a rapid, reproducible and discriminative method for characterization of Staphylococci which is easy to perform and interpret.
DOI
-
Keywords
Staphylococcus aureus, coagulase-negative staphylococci (CNS), protein profiles, typing.
First Page
145
Last Page
151
Recommended Citation
SAÇILIK, S. C, OSMANAĞAOĞLU, Ö, SAYAR, A. H, & ÇÖKMÜŞ, C (2001). Availability of Use of Total Extracellular Proteins in SDS-PAGE for Typing Staphylococcus aureus and Coagulase-Negative Staphylococci. Turkish Journal of Biology 25 (2): 145-151. https://doi.org/-
