Turkish Journal of Biology
DOI
-
Abstract
Buchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a rapid and simple three-step purification procedure including freeze drying, silicic acid adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity increased about 111 and 2,500 fold, respectively. It was determined that the adsorption of buchnericin LB to silicic acid and cation-exchange chromatography was dependent on the pH of the suspending environment. The molecular weight of buchnericin LB was determined to be about 4.0 kDa by tricine SDS-PAGE.
Keywords
Bacteriocin, buchnericin LB, Lactobacillus bunchneri LB, purification, silicic acid, cation- exchange chromatography.
First Page
59
Last Page
65
Recommended Citation
YILDIRIM, METİN (2001) "Purification of Buchnericin LB Produced by Lactobacillus buchneri LB," Turkish Journal of Biology: Vol. 25: No. 1, Article 7. Available at: https://journals.tubitak.gov.tr/biology/vol25/iss1/7
